Box contains contains 1 ml of 2xMasterMix, 25 mM MgCl2, nuclease free water and DMSO. 2XMasterMix is premixed with sample loading dye for immediate loading after the PCR reaction..
Stable at room temperature. Long term storage at -20^oC required..
Applications PCR amplification of DNA fragments up to 10 kb DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR..
HotStart 2xTaq DNA Pol Master Mix,1ML, P03-CR03SM-1ML
Contains 1 ml of 2xMasterMix, 25 mM MgCl2, nuclease free water and DMSO. 2XMasterMix is premixed with sample loading dye for immediate loading after the PCR reaction..
HotStart PCR for specific amplification. Comparable with the Platinum Taq polymerase. Good for TA cloning..
Dye Minus HotSatrt 2X Master Mix is also available upon request..
Applications PCR amplification of DNA fragments up to 10 kb DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.
10×PCR Buffer 750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM KCl, 50 mM (NH4)2SO4, 15mM MgCl2, 0.5% NP-40.
Unit definition One unit is the amount of enzyme required to catalyze the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74°C under assay conditions..
Applications PCR amplification of DNA fragments up to 5 kb DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.